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The graph above shows two definitions of the IC50. The relative IC50 is by far the most common definition, and the adjective relative is usually omitted. It is the concentration required to bring the curve down to point half way between the top and bottom plateaus of the curve. The NS values are totally ignored with this definition of IC50.Relevance of MIC50 and MIC90 determination?Relevance of MIC50 and MIC90 determination? Please let me know which value is more reliable for minimum inhibitory concentration determination. What is the basic difference in both?How can I determine an EC90 (or any EC value other than EC50)?(This equation was derived by starting with the equation that defines percent response F, from EC50, H, and concentration, and then rearranging to solve for the concentration (ECF).) If you know the EC50 and Hill slope (H), you can easily compute the EC80 or EC10 or any other value you want.

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